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a303 456a  (Bethyl)


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    Bethyl a303 456a
    A303 456a, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ufl1/UFL1+Antibody/pm41886456-322-10-8
    Average 93 stars, based on 33 article reviews
    a303 456a - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: The Epstein-Barr virus deubiquitinase BPLF1 regulates stress-induced ribosome UFMylation and reticulophagy.
    Article Snippet: .. Mouse monoclonal antibodies: anti-ACTB/β-actin clone AC15 (1:5000; Sigma-Aldrich, A5441); anti-GAPDH (1:10000; Millipore, CB1001); anti-TUBA/tubulin (1:5000; Millipore, CP06); anti-FLAG (1:10000; Sigma-Aldrich, F3165); anti-S Tag (1:2000; Millipore 71,549–3); anti-HA Tag, (1:2000; Sigma-Aldrich, H9658); anti-RPN1 (1:1000; Santa Cruz Biotechnology, sc -48,367); anti-EIF4G1 Clone 2A9 (1:1000; Abnova, H00001981-M10); anti-ubiquitin, (1:1000; Santa Cruz Biotechnology, sc8017); anti-MYC Tag, clone 4A6, agarose conjugate, (Millipore; 16–219); anti-EBV-BMRF1 (1:10000; Dr. Jaap M. Middeldorp, VU University Medical Center, Amsterdam, NL); Mouse polyclonal antibodies: anti- GFP(B-2) (1:1000; Santa Cruz Biotechnology, sc-9996); antiFLAG, (1:5000; Sigma-Aldrich, F7425); anti-GFP (1:3000; Abcam, ab290); anti-GFP (1:3000; Abcam, ab290); rabbit monoclonal antibodies: anti-UFC1, (1:5000; Abcam, ab189251); anti-UFM1 (1:3000; Abcam, ab109305); antiMYC Tag (1:1000; Cell Signaling Technology, 2278); antiUFM1 (1:3000; Abcam, ab109305); anti-UFC1, (1:5000; Abcam, ab189251); anti-UFSP2 (1:3000; Abcam, ab185965); anti-RPS10 (1:2000; Abcam, ab151550); anti-RPS20 (1:2000; Abcam, ab133776); rabbit polyclonal antibodies anti-ZNF598 (1:1000; Invitrogen, PA559777); anti-LTN1 (1:1000; ProteinTech 28,452–1-AP); anti-UFL1 (1:5000; Bethyl Laboratories, A303-456A); anti-CDK5RAP3 (1:3000; ProteinTech 11,007–1-AP); anti-DDRGK1 (1:3000; Thermo Fisher Scientific, PA5–100467); anti-histone H3 (1:2000; Millipore, 07–690); anti-SEC63 (1:2000; Bethyl Laboratories, A305-084A); anti-SRPRB (1:2000; Bethyl Laboratories, A305440A); anti-SRP68 (1:2000; Bethyl Laboratories, A303-955A); anti-RPL26, (1:2000; Abcam, ab59567); anti-SEC61B (1:10000; Thermo Fisher Scientific, PA3–015); anti-CYB5R3 (1:2000; ProteinTech 10,894–1-AP); anti-LC3B (1:1000; SigmaAldrich, L7543); anti-SUMO2 + 3 (1:1000; Abcam, ab3742); anti-FLAG M2 affinity gel (Sigma-Aldrich, A2220); S-Protein Agarose, (Novagen 69,704); GammaBind Plus Sepharose (Cytiva 17,088,601); Rabbit IgG isotype control (Abcam, ab172730); Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor 647 Conjugated (1:1000; Thermo Fisher Scientific, A21245). ..

    Article Title: The Epstein-Barr virus deubiquitinase BPLF1 regulates stress-induced ribosome UFMylation and reticulophagy
    Article Snippet: .. Mouse monoclonal antibodies: anti-ACTB/β-actin clone AC-15 (1:5000; Sigma-Aldrich, A5441); anti-GAPDH (1:10000; Millipore, CB1001); anti-TUBA/tubulin (1:5000; Millipore, CP06); anti-FLAG (1:10000; Sigma-Aldrich, F3165); anti-S Tag (1:2000; Millipore 71,549–3); anti-HA Tag, (1:2000; Sigma-Aldrich, H9658); anti-RPN1 (1:1000; Santa Cruz Biotechnology, sc -48,367); anti-EIF4G1 Clone 2A9 (1:1000; Abnova, H00001981-M10); anti-ubiquitin, (1:1000; Santa Cruz Biotechnology, sc8017); anti-MYC Tag, clone 4A6, agarose conjugate, (Millipore; 16–219); anti-EBV-BMRF1 (1:10000; Dr. Jaap M. Middeldorp, VU University Medical Center, Amsterdam, NL); Mouse polyclonal antibodies: anti-GFP(B-2) (1:1000; Santa Cruz Biotechnology, sc-9996); anti-FLAG, (1:5000; Sigma-Aldrich, F7425); anti-GFP (1:3000; Abcam, ab290); anti-GFP (1:3000; Abcam, ab290); rabbit monoclonal antibodies: anti-UFC1, (1:5000; Abcam, ab189251); anti-UFM1 (1:3000; Abcam, ab109305); anti-MYC Tag (1:1000; Cell Signaling Technology, 2278); anti-UFM1 (1:3000; Abcam, ab109305); anti-UFC1, (1:5000; Abcam, ab189251); anti-UFSP2 (1:3000; Abcam, ab185965); anti-RPS10 (1:2000; Abcam, ab151550); anti-RPS20 (1:2000; Abcam, ab133776); rabbit polyclonal antibodies anti-ZNF598 (1:1000; Invitrogen, PA559777); anti-LTN1 (1:1000; ProteinTech 28,452–1-AP); anti-UFL1 (1:5000; Bethyl Laboratories, A303-456A); anti-CDK5RAP3 (1:3000; ProteinTech 11,007–1-AP); anti-DDRGK1 (1:3000; Thermo Fisher Scientific, PA5–100467); anti-histone H3 (1:2000; Millipore, 07–690); anti-SEC63 (1:2000; Bethyl Laboratories, A305-084A); anti-SRPRB (1:2000; Bethyl Laboratories, A305-440A); anti-SRP68 (1:2000; Bethyl Laboratories, A303-955A); anti-RPL26, (1:2000; Abcam, ab59567); anti-SEC61B (1:10000; Thermo Fisher Scientific, PA3–015); anti-CYB5R3 (1:2000; ProteinTech 10,894–1-AP); anti-LC3B (1:1000; Sigma-Aldrich, L7543); anti-SUMO2 + 3 (1:1000; Abcam, ab3742); anti-FLAG M2 affinity gel (Sigma-Aldrich, A2220); S-Protein Agarose, (Novagen 69,704); GammaBind Plus Sepharose (Cytiva 17,088,601); Rabbit IgG isotype control (Abcam, ab172730); Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor 647 Conjugated (1:1000; Thermo Fisher Scientific, A21245). ..

    Control:

    Article Title: The Epstein-Barr virus deubiquitinase BPLF1 regulates stress-induced ribosome UFMylation and reticulophagy.
    Article Snippet: .. Mouse monoclonal antibodies: anti-ACTB/β-actin clone AC15 (1:5000; Sigma-Aldrich, A5441); anti-GAPDH (1:10000; Millipore, CB1001); anti-TUBA/tubulin (1:5000; Millipore, CP06); anti-FLAG (1:10000; Sigma-Aldrich, F3165); anti-S Tag (1:2000; Millipore 71,549–3); anti-HA Tag, (1:2000; Sigma-Aldrich, H9658); anti-RPN1 (1:1000; Santa Cruz Biotechnology, sc -48,367); anti-EIF4G1 Clone 2A9 (1:1000; Abnova, H00001981-M10); anti-ubiquitin, (1:1000; Santa Cruz Biotechnology, sc8017); anti-MYC Tag, clone 4A6, agarose conjugate, (Millipore; 16–219); anti-EBV-BMRF1 (1:10000; Dr. Jaap M. Middeldorp, VU University Medical Center, Amsterdam, NL); Mouse polyclonal antibodies: anti- GFP(B-2) (1:1000; Santa Cruz Biotechnology, sc-9996); antiFLAG, (1:5000; Sigma-Aldrich, F7425); anti-GFP (1:3000; Abcam, ab290); anti-GFP (1:3000; Abcam, ab290); rabbit monoclonal antibodies: anti-UFC1, (1:5000; Abcam, ab189251); anti-UFM1 (1:3000; Abcam, ab109305); antiMYC Tag (1:1000; Cell Signaling Technology, 2278); antiUFM1 (1:3000; Abcam, ab109305); anti-UFC1, (1:5000; Abcam, ab189251); anti-UFSP2 (1:3000; Abcam, ab185965); anti-RPS10 (1:2000; Abcam, ab151550); anti-RPS20 (1:2000; Abcam, ab133776); rabbit polyclonal antibodies anti-ZNF598 (1:1000; Invitrogen, PA559777); anti-LTN1 (1:1000; ProteinTech 28,452–1-AP); anti-UFL1 (1:5000; Bethyl Laboratories, A303-456A); anti-CDK5RAP3 (1:3000; ProteinTech 11,007–1-AP); anti-DDRGK1 (1:3000; Thermo Fisher Scientific, PA5–100467); anti-histone H3 (1:2000; Millipore, 07–690); anti-SEC63 (1:2000; Bethyl Laboratories, A305-084A); anti-SRPRB (1:2000; Bethyl Laboratories, A305440A); anti-SRP68 (1:2000; Bethyl Laboratories, A303-955A); anti-RPL26, (1:2000; Abcam, ab59567); anti-SEC61B (1:10000; Thermo Fisher Scientific, PA3–015); anti-CYB5R3 (1:2000; ProteinTech 10,894–1-AP); anti-LC3B (1:1000; SigmaAldrich, L7543); anti-SUMO2 + 3 (1:1000; Abcam, ab3742); anti-FLAG M2 affinity gel (Sigma-Aldrich, A2220); S-Protein Agarose, (Novagen 69,704); GammaBind Plus Sepharose (Cytiva 17,088,601); Rabbit IgG isotype control (Abcam, ab172730); Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor 647 Conjugated (1:1000; Thermo Fisher Scientific, A21245). ..

    Article Title: The Epstein-Barr virus deubiquitinase BPLF1 regulates stress-induced ribosome UFMylation and reticulophagy
    Article Snippet: .. Mouse monoclonal antibodies: anti-ACTB/β-actin clone AC-15 (1:5000; Sigma-Aldrich, A5441); anti-GAPDH (1:10000; Millipore, CB1001); anti-TUBA/tubulin (1:5000; Millipore, CP06); anti-FLAG (1:10000; Sigma-Aldrich, F3165); anti-S Tag (1:2000; Millipore 71,549–3); anti-HA Tag, (1:2000; Sigma-Aldrich, H9658); anti-RPN1 (1:1000; Santa Cruz Biotechnology, sc -48,367); anti-EIF4G1 Clone 2A9 (1:1000; Abnova, H00001981-M10); anti-ubiquitin, (1:1000; Santa Cruz Biotechnology, sc8017); anti-MYC Tag, clone 4A6, agarose conjugate, (Millipore; 16–219); anti-EBV-BMRF1 (1:10000; Dr. Jaap M. Middeldorp, VU University Medical Center, Amsterdam, NL); Mouse polyclonal antibodies: anti-GFP(B-2) (1:1000; Santa Cruz Biotechnology, sc-9996); anti-FLAG, (1:5000; Sigma-Aldrich, F7425); anti-GFP (1:3000; Abcam, ab290); anti-GFP (1:3000; Abcam, ab290); rabbit monoclonal antibodies: anti-UFC1, (1:5000; Abcam, ab189251); anti-UFM1 (1:3000; Abcam, ab109305); anti-MYC Tag (1:1000; Cell Signaling Technology, 2278); anti-UFM1 (1:3000; Abcam, ab109305); anti-UFC1, (1:5000; Abcam, ab189251); anti-UFSP2 (1:3000; Abcam, ab185965); anti-RPS10 (1:2000; Abcam, ab151550); anti-RPS20 (1:2000; Abcam, ab133776); rabbit polyclonal antibodies anti-ZNF598 (1:1000; Invitrogen, PA559777); anti-LTN1 (1:1000; ProteinTech 28,452–1-AP); anti-UFL1 (1:5000; Bethyl Laboratories, A303-456A); anti-CDK5RAP3 (1:3000; ProteinTech 11,007–1-AP); anti-DDRGK1 (1:3000; Thermo Fisher Scientific, PA5–100467); anti-histone H3 (1:2000; Millipore, 07–690); anti-SEC63 (1:2000; Bethyl Laboratories, A305-084A); anti-SRPRB (1:2000; Bethyl Laboratories, A305-440A); anti-SRP68 (1:2000; Bethyl Laboratories, A303-955A); anti-RPL26, (1:2000; Abcam, ab59567); anti-SEC61B (1:10000; Thermo Fisher Scientific, PA3–015); anti-CYB5R3 (1:2000; ProteinTech 10,894–1-AP); anti-LC3B (1:1000; Sigma-Aldrich, L7543); anti-SUMO2 + 3 (1:1000; Abcam, ab3742); anti-FLAG M2 affinity gel (Sigma-Aldrich, A2220); S-Protein Agarose, (Novagen 69,704); GammaBind Plus Sepharose (Cytiva 17,088,601); Rabbit IgG isotype control (Abcam, ab172730); Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor 647 Conjugated (1:1000; Thermo Fisher Scientific, A21245). ..

    Western Blot:

    Article Title: PARP1 UFMylation ensures the stability of stalled replication forks
    Article Snippet: .. Immunoblotting, immunoprecipitation, pull-down assays and immunofluorescence First antibodies: anti-HA (A190-208A), anti-MYC (A190-205A), anti-UFL1 (A303455A, A303-456A), anti-PARP1 (A301-375A), anti-CHK1 (A300-298A), anti-BRCA2 (A303-434A), anti-RPA32 (A300-244A), and anti-pRPA32 (S33) (A300-246A) were purchased from Bethyl. .. Anti-PAR (ab14459), anti-BrdU (ab6326), anti-H3 (ab1791), and anti-MRE11 (ab214) was purchased from Abcam.

    Immunoprecipitation:

    Article Title: PARP1 UFMylation ensures the stability of stalled replication forks
    Article Snippet: .. Immunoblotting, immunoprecipitation, pull-down assays and immunofluorescence First antibodies: anti-HA (A190-208A), anti-MYC (A190-205A), anti-UFL1 (A303455A, A303-456A), anti-PARP1 (A301-375A), anti-CHK1 (A300-298A), anti-BRCA2 (A303-434A), anti-RPA32 (A300-244A), and anti-pRPA32 (S33) (A300-246A) were purchased from Bethyl. .. Anti-PAR (ab14459), anti-BrdU (ab6326), anti-H3 (ab1791), and anti-MRE11 (ab214) was purchased from Abcam.

    Immunofluorescence:

    Article Title: PARP1 UFMylation ensures the stability of stalled replication forks
    Article Snippet: .. Immunoblotting, immunoprecipitation, pull-down assays and immunofluorescence First antibodies: anti-HA (A190-208A), anti-MYC (A190-205A), anti-UFL1 (A303455A, A303-456A), anti-PARP1 (A301-375A), anti-CHK1 (A300-298A), anti-BRCA2 (A303-434A), anti-RPA32 (A300-244A), and anti-pRPA32 (S33) (A300-246A) were purchased from Bethyl. .. Anti-PAR (ab14459), anti-BrdU (ab6326), anti-H3 (ab1791), and anti-MRE11 (ab214) was purchased from Abcam.

    other:

    Article Title: VCP/p97 UFMylation stabilizes BECN1 and facilitates the initiation of autophagy.
    Article Snippet: Macroautophagy/autophagy is essential for the degradation and recycling of cytoplasmic materials.. The initiation of this process is determined by phosphatidylinositol-3-kinase (PtdIns3K) complex, which is regulated by factor BECN1 (beclin 1).. UFMylation is a novel ubiquitin-like modification that has been demonstrated to modulate several cellular activities.



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    a , b TNBC patient-derived xenografts (PDXs) were subcutaneously implanted into nude mice. Saline, PDAU or NP (15 mg/kg) was injected intraperitoneally every 2 days when tumor volume reached 100 mm 3 . Mice were then treated with saline or cisplatin (5 mg/kg weekly, n = 6). Tumors were collected ( a ), and tumor weights were measured ( b ). Results represent the mean ± SD from six mice. c Cell lysates from PDXs treated with saline, PDAU or NP were subjected to immunoprecipitation with IgG, anti-UFL1 antibodies. The immunoprecipitates were blotted with the indicated antibodies. d Cell lysates from PDXs treated with saline, PDAU or NP were subjected to western blot with the indicated antibodies. e Representative images of H&E, Ki67 and cleaved PARP1 staining in xenograft models from ( a ). Scale bars, 50 μm. Data were analyzed by two-sided one-way ANOVA in ( b ). Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Targeting the UFL1-AKT cascade suppresses triple-negative breast cancer progression

    doi: 10.1038/s41467-026-68493-2

    Figure Lengend Snippet: a , b TNBC patient-derived xenografts (PDXs) were subcutaneously implanted into nude mice. Saline, PDAU or NP (15 mg/kg) was injected intraperitoneally every 2 days when tumor volume reached 100 mm 3 . Mice were then treated with saline or cisplatin (5 mg/kg weekly, n = 6). Tumors were collected ( a ), and tumor weights were measured ( b ). Results represent the mean ± SD from six mice. c Cell lysates from PDXs treated with saline, PDAU or NP were subjected to immunoprecipitation with IgG, anti-UFL1 antibodies. The immunoprecipitates were blotted with the indicated antibodies. d Cell lysates from PDXs treated with saline, PDAU or NP were subjected to western blot with the indicated antibodies. e Representative images of H&E, Ki67 and cleaved PARP1 staining in xenograft models from ( a ). Scale bars, 50 μm. Data were analyzed by two-sided one-way ANOVA in ( b ). Source data are provided as a Source Data file.

    Article Snippet: Anti-UFL1 (A303-456A, dilution: 1:1000) antibodies were purchased from BETHYL.

    Techniques: Derivative Assay, Saline, Injection, Immunoprecipitation, Western Blot, Staining